goat anti human kappa light chain secondary antibody  (Bio-Rad)

Journal: Nature Communications

Article Title: Filamentous fungus-produced human monoclonal antibody provides protection against SARS-CoV-2 in hamster and non-human primate models

doi: 10.1038/s41467-024-46443-0

Figure Lengend Snippet: Stained SDS gel ( A ) and Western ( B ) analysis of the fermentation culture supernatants of the C1 strain expressing HuMab 87G7. Lane 1, HEK293T cell-produced 87G7, lanes 2–5, supernatant samples from days 3–6 of the culture. Reduced and non-reduced samples are shown as indicated above the panels. The antibody used for HC detection was Anti-human IgG F(c) Goat Polyclonal Antibody (IRDye700DX) and the one used for LC was Goat anti-Human Kappa Light Chain Secondary Antibody (DyLight 800). C C1-produced HuMab 87G7 after purification in stained SDS gel analysis. Lane 1, reduced (R) C1-produced HuMab 87G7; lane 2, non-reduced (N-R) C1-produced HuMab 87G7; lane 3, non-reduced (N-R) HEK293T cell-produced HuMab 87G7. The experiment was performed twice, data from a representative experiment are shown.

Article Snippet: The antibody used for HC detection was anti-human IgG F(c) Goat Polyclonal Antibody (IRDye700DX, Li-Cor, Lincoln, Nebraska, USA) and the with Goat anti-human Kappa Light Chain secondary antibody (DyLight 800, Li-Cor) used for LC detection.

Techniques: Staining, SDS-Gel, Western Blot, Expressing, Produced, Purification

Journal: Acta neuropathologica communications

Article Title: Molecular signatures of inherited and acquired sporadic late onset nemaline myopathies.

doi: 10.1186/s40478-023-01518-9

Figure Lengend Snippet: Fig. 4 Analysis of immunoglobulin expression and autoimmune markers in inherited and sporadic late-onset nemaline myopathies (A) Proteomic analysis showed an increased abundance of IgG heavy chains and both kappa and lambda light chains in nemaline rod areas in sporadic late-onset nemaline myopathy (SLONM) relative to inherited nemaline myopathies (iNM). There was also a trend towards an increased abundance of immunoglobulin chains in the nemaline rod areas of SLONM relative to rod-free areas. Striped bars indicate immunoglobulin chains that were only detected in one of the groups of samples compared *p < 0.05. (B) Immunofluorescent staining demonstrated accumulation of kappa light chains in atrophic fibers in 38% of SLONM biopsies (bottom row), but not in iNM (top row). Scale bar = 400 µm. (C) Immunoglobulin genes were also differentially expressed between SLONM and iNM. (D) Immunostaining for Major Histocompatibility Antigen-I (MHC-I) showed reactivity in SLONM biopsies, occurring predominantly in atrophic fibers (first panel, SLONM with monoclonal gammopathy; second panel, SLONM without monoclonal gammopathy). Biopsies from patients with inherited nemaline myopathy showed no MHC-I reactivity or occasional fibers faintly reactive for MHC-I (third panel, adult patient with ACTA1 nemaline myopathy). MHC-I reactivity was absent in healthy control muscle (fourth panel). Scale bar = 100 µm

Article Snippet: Frozen skeletal muscle sections (8 μm) were post-fixed in 4% paraformaldehyde for 5 min at room temperature prior to immunostaining with laminin (L0663, Sigma, dilution 1:250) and human kappa light chain (NBP269235, Novus Biologicals, Centennial, CO, dilution 1:200) antibodies as previously described [18].

Techniques: Expressing, Staining, Immunostaining, Control